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. 2021 Aug 25;27(20):5669–5680. doi: 10.1158/1078-0432.CCR-21-0312

Figure 5.

Figure 5. TOP2B expression is associated with tumor growth phenotype in gliomas. A, Violin plot of GSVA analysis of genes related to cell-cycle genes comparing their expression on GBM specimens with high versus low TOP2B. B, Cell viability for TS543 glioma cells transduced with TET-ON inducible TOP2B shRNA treated with or without doxycycline, two representative clones are shown. Experiments were performed in five clones. C, Fluorescent microscopy and overall survival analysis following intracranial injection of BT142 TET-ON inducible TOP2B shRNA clones and treatment doxycycline or sucrose in drinking water (15 mice per group), and a similar survival analysis performed with BT142 untransduced cells (BT142wt). D, IHC analysis for TOP2B, PDGFRA, MYC and, hematoxylin & eosin staining for cellular density using four mice bearing intracranial gliomas BT142 TET-ON with inducible TOP2B shRNA treated with or without doxycycline in the drinking water. Quantification for total TOP2B, PDGFRA, and MYC signal on entire tumors.

TOP2B expression is associated with tumor growth phenotype in gliomas. A, Violin plot of GSVA analysis of genes related to cell-cycle genes comparing their expression on GBM specimens with high versus low TOP2B. B, Cell viability for TS543 glioma cells transduced with TET-ON inducible TOP2B shRNA treated with or without doxycycline, two representative clones are shown. Experiments were performed in five clones. C, Fluorescent microscopy and overall survival analysis following intracranial injection of BT142 TET-ON inducible TOP2B shRNA clones and treatment doxycycline or sucrose in drinking water (15 mice per group), and a similar survival analysis performed with BT142 untransduced cells (BT142wt). D, IHC analysis for TOP2B, PDGFRA, MYC and, hematoxylin & eosin staining for cellular density using four mice bearing intracranial gliomas BT142 TET-ON with inducible TOP2B shRNA treated with or without doxycycline in the drinking water. Quantification for total TOP2B, PDGFRA, and MYC signal on entire tumors.