Figure 2. SEC HPLC analyses of DOTA-anti-CEA-Fab’-LND and DOTA-anti-CEA-LND.
A. DOTA-anti-CEA Fab’-azide. B. DOTA-anti-CEA Fab’-azide clicked to DSPE-PEG2000-DBCO LND. C. Immunoreactivity of DOTA-anti-CEA Fab’-azide clicked to DSPE-PEG2000-DBCO LND after addition of 2-fold excess CEA. D. DOTA-anti-CEA-azide. E. DOTA-anti-CEA-azide clicked to DSPE-PEG2000-DBCO LND. F. Immunoreactivity of DOTA-anti-CEA-azide-DSPE-PEG2000-DBCO LND after addition of two-fold excess of CEA. Red= 214 nm. Blue- 240 nm.