REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
FectoPRO	Polyplus	Cat# 101000007
Expi293 Expression Media	Thermo Scientific	Cat# A14635
Opti-MEM™ Reduced Serum Media	Thermo Scientific	Cat# 31985-070
IMAC Sepharose 6 Fast Flow	GE Healthcare	Cat# 17092109
Tris Base	Thermo Scientific	Cat# BP152-5
Sodium Chloride	Thermo Scientific	Cat# S271-10
Imidazole	ACROS	Cat# 301870025
HEPES	Sigma	Cat# H3375
Critical commercial assays
Spin Miniprep Kit	Qiagen	Cat# 27106
Hispeed Plasmid Maxi Kit	Qiagen	Cat# 12663
HisTrap Fast Flow	GE Healthcare	Cat# 17-0921-09
Ni-NTA Agarose	Thermo Scientific	Cat# R90115
Deposited data
Cryo-EM structure of the SARS-CoV-2 spike glycoprotein Omicron B.1.1.529 variant	This paper	PDB: 7THK
Cryo-EM map of the SARS-CoV-2 spike glycoprotein Omicron B.1.1.529 variant	This paper	EMDB: EMD-25896
Experimental models: Cell lines
Expi293F Cells	Thermo Scientific	Cat# A14527
Recombinant DNA
pCMV3-B.1.1.1529 spike	Li et al., 2021	N/A
pαH vector	https://www.addgene.org/154754/	Cat# 154754
Software and algorithms
Coot	Emsley and Cowtan, 2004	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot
cryoSPARC	Punjani et al., 2017	https://cryosparc.com
Leginon	Suloway et al., 2005	https://sbgrid.org/software/titles/leginon
Molprobity	Davis et al., 2004	http://molprobity.biochem.duke.edu
Phenix	Adams et al., 2010	https://www.phenix-online.org
The PyMOL Molecular Graphics System, Version 2.0	Schrödinger, LLC	https://pymol.org/2/support.html#page-top
RELION	Scheres, 2012	https://www3.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page
DeepEMhancer	Sanchez-Garcia et al., 2021	https://github.com/rsanchezgarc/deepEMhancer
EMRinger	Barad et al., 2015	https://github.com/fraser-lab/EMRinger
UCSF Chimera	Pettersen et al., 2004	https://www.cgl.ucsf.edu/chimera/
UCSF Chimera X	Pettersen et al., 2021	https://www.cgl.ucsf.edu/chimerax/
GraphPad Prism Software	GraphPad Prism Software, Inc.	N/A
PDBePISA	Krissinel and Henrick, 2007	https://www.ebi.ac.uk/pdbe/pisa/
Python v3.8.3	Python	https://www.python.org/
The R Project for Statistical Computing	R Core Team	https://www.r-project.org/
R bio3d package	Grant et al., 2006	http://thegrantlab.org/bio3d/