Fig. 3.

Thickening of blood vessels and reduction of vascular mural cell coverage in aldh2.1^−/−retinae. A: Representative light microscopy images of zebrafish retinae prepared with trypsin digestion and hematoxylin staining. Red Arrow: erythrocyte, black arrow: pericyte, white arrow: endothelial cell, black scale bar: 100 μm. B-E: Quantification of vascular parameters. B: Increased vessel diameter, C: Increased capillary area, D: Unaltered endothelial cell count and E: Reduced vascular mural cell count. n = 37–39. Statistical analysis was done via Student's t-test, ns = not significant, *p < 0.05, ***p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)