Fig. 5.

aldh2.1^−/−mutants exhibited aggravated stress-signaling and an impairment of glycolysis and gluconeogenesis. A: Bar diagram for highest, significantly increased or decreased pathways in a Kyoto-encyclopedia of gene and genomes (KEGG) pathways analysis in aldh2.1^−/− larvae. B-E: Gene set enrichment analysis (GSEA) plots for VEGF (B), MAPK (C), glycolysis/gluconeogenesis (D), pyruvate metabolism (E) pathways. RNA-seq was done with mRNA in 120 hpf zebrafish larvae, quantification via normalized enrichment score (NES), n = 5 clutches, 50 larvae per clutch, ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001.