Fig. 6.

Inhibition of glucose-6-phosphatase/glucokinase expression and alteration of JNK and p38 MAPK expression in aldh2.1^−/−mutants. A-F: Expression of MAPK family members. ERK1 (A), ERK5 (B) and p38γ (E) were unaltered in aldh2.1^−/− larvae. JNK1 (C) is significantly upregulated; p38α (F) and p38β (D) were significantly downregulated in aldh2.1^−/− animals. G-L: Expression of key regulatory genes of glycolysis and gluconeogenesis. Strongest down regulation in aldh2.1^−/− larvae is shown for g6pc (G) and gck (L). g6pd (H), pck1 (I) and hk2 (K) were also significantly down regulated. hk1 (J) gene expression was unchanged. Expression was quantified via RT-qPCR with 96 hpf zebrafish larvae and normalized to arnt2, n = 5–8 clutches, 50 larvae per clutch. Statistical analysis was done via Student's t-test, ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.