Fig. 3.

The effect of anti-MUC1 mAb onMUC1 mRNA (A), MUC1 glycoprotein expression in cell lysates (B) and culture medium (C) of DLD-1 and HT-29 colon cancer cells. The cancer cells were incubated for 24 h with 5 μg/mL of anti-MUC1. mRNA was assessed by RT-PCR. The result is presented as a relative fold change in mRNA expression of gene in comparison of gene in control where expression was set as 1. ±SD are the mean of triplicate cultures. *P < 0.05; **P < 0.01. MUC1 glycoprotein expression was assessed by ELISA tests. The results are expressed as absorbance at 405 nm after reactivity with anti-MUC1 monoclonal antibody. Values ±SD are the mean from three independent assays. *P < 0.05, **P < 0.01, ***P < 0.001.