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. 2022 Feb 1;18:101348. doi: 10.1016/j.tranon.2022.101348

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© 2022 Published by Elsevier Inc.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig 9 — The effect of PtPz4, PtPz6, cisPt, and anti-MUC1 on cancer related Tn antigen (detected by VVA lectin) (A), T antigen (detected by PNA lectin) (B), sialyl Tn (detected by SNA lectin) (C), and sialyl T (detected by MAAII lectin) (D) in DLD-1 and HT-29 colon cancer cells. The cells were incubated for 24 h with PtPz4 (10 μM), PtPz6 (10 μM), cisPt (10 μM), anti-MUC1 (5 μg/mL), PtPz4 + anti-MUC1 (10 μM + 5 μg/mL), PtPz6 + anti-MUC1 (10 μM + 5 μg/mL), and cisPt + anti-MUC1 (10 μM + 5 μg/mL). The relative expressions of antigens in cell lysates and culture medium were assessed by ELISA tests. The results are expressed as absorbance at 405 nm after reactivity with biotinylated lectins. Values ±SD are the mean from three independent assays. *P < 0.05, **P < < 0.01, ***P < 0.001 compared to the untreated control; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 compared to PtPz4 monotherapy; ^ΔP < 0.05, ^ΔΔP < 0.01, ^ΔΔΔP < 0.001 compared to PtPz6 monotherapy; ^†P < 0.05, ^††P < 0.01, ^†††P < 0.001 compared to cisPt monotherapy; ^‡P < 0.05, ^‡‡P < 0.01, ^‡‡‡P < 0.001 compared to anti-MUC1 monotherapy.

Fig 9 — The effect of PtPz4, PtPz6, cisPt, and anti-MUC1 on cancer related Tn antigen (detected by VVA lectin) (A), T antigen (detected by PNA lectin) (B), sialyl Tn (detected by SNA lectin) (C), and sialyl T (detected by MAAII lectin) (D) in DLD-1 and HT-29 colon cancer cells. The cells were incubated for 24 h with PtPz4 (10 μM), PtPz6 (10 μM), cisPt (10 μM), anti-MUC1 (5 μg/mL), PtPz4 + anti-MUC1 (10 μM + 5 μg/mL), PtPz6 + anti-MUC1 (10 μM + 5 μg/mL), and cisPt + anti-MUC1 (10 μM + 5 μg/mL). The relative expressions of antigens in cell lysates and culture medium were assessed by ELISA tests. The results are expressed as absorbance at 405 nm after reactivity with biotinylated lectins. Values ±SD are the mean from three independent assays. *P < 0.05, **P < < 0.01, ***P < 0.001 compared to the untreated control; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 compared to PtPz4 monotherapy; ^ΔP < 0.05, ^ΔΔP < 0.01, ^ΔΔΔP < 0.001 compared to PtPz6 monotherapy; ^†P < 0.05, ^††P < 0.01, ^†††P < 0.001 compared to cisPt monotherapy; ^‡P < 0.05, ^‡‡P < 0.01, ^‡‡‡P < 0.001 compared to anti-MUC1 monotherapy.