Skip to main content
. 2022 Jan 24;12:823935. doi: 10.3389/fimmu.2021.823935

Figure 1.

Figure 1

Renal denervation inhibits the fibrotic phenotype during UUO and UIRI. C57BL/6 mice were subjected to UUO or UIRI manipulation 2 days after renal denervation (DNx) or sham operation in the left kidneys. (A, B) Representative histology of kidney sections from sham-operated, UUO/UIRI mice, and DNx+UUO/UIRI mice stained with periodic acid–Schiff, Sirius Red, and Masson’s trichrome stain. Scale bar, 50μm. (C, D) Representative Western blot of fibronectin (FN), Collagen I (Col 1), α-SMA, and Tyrosine Hydroxylase (TH) in the kidney tissue of sham-operated, UUO/UIRI mice, and DNx+UUO/UIRI mice with densitometry analysis (E, F). (G, H) Representative mRNA expressions of FN, Col 1, and α-SMA in the kidney tissue of sham-operated, UUO/UIRI mice, and DNx+UUO/UIRI mice using qRT-PCR analysis. n=3–7 in each group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Bars represented means ± SEM.