Figure 2.

The morphology of Neu in 2D and 3D culture systems. (A) Morphology of Cellmatrix Type I, I-PC collagen and I-AC and Collagen type I in a static state or vibrational under light microscope. (B) Toxicity testing was evaluated by the MTT assay. Mouse bone marrow-derived Neu were embedded in Cellmatrix Type I and cultured for 24 h. MTT reagent was used to detect the survival rate of the cells. (C) The morphology of mouse peripheral blood Neu representative light microscopy photographs (600x). (D) Representative fluorescence micrograph of human peripheral blood Neu dyed by SYTO 13 (stain DNA in both live and dead eukaryotic cells) during 6 days in 2D and 3D culture systems with or without the inhibition of apoptosis z-vad-fmk. Error bars represent mean ± S.D. n = 3, ns, not statistically significantly different by One-way ANOVA.