Figure 4.
The activity of Neu in 2D and 3D culture systems. (A) Survival rate was evaluated by the MTT assay. (B) Percentage of the absorbance value relative to the cells on day 1 was used to indicate the active cells. (C) DNA integrity was determined by electrophoresis of DNA isolated from cultured mouse Neu for 1-6 days. Each slot was loaded with 50 μg of genomic DNA (corresponding to 5x105 Neu used for preparation). Percentage of PI positive in the total mouse (D) and human (E) peripheral blood Neu which were dyed by SYTO 13 during 6 days in 2D and 3D culture systems with or without the inhibition of apoptosis z-vad-fmk was used to indicate the dead cells. Data from one of three experiments performed are shown. Error bars represent mean ± S.D. n = 3, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not statistically significantly different by Two way ANOVA.