Figure 4.

Increased numbers of microglia/macrophages in the optic nerve. (A) Optic nerve sections were stained with Iba1 (red, microglia/macrophages) and Tmem119 (green, microglia) at six and after eight weeks. White arrows indicate co-localized Tmem119⁺ and Iba1⁺ cells. (B) The number Iba1⁺ cells was significantly increased in the OSE group after six weeks. The staining after eight weeks still displayed an increase of Iba1⁺ cells among the OSE group. (C) Tmem119⁺ cells were also increased after six weeks. No difference of the number of Tmem119⁺ cells was measurable between both groups at eight weeks. (D) A higher number of microglia (Tmem119⁺ and Iba1⁺) was observed at six weeks. This elevation was still noted in the OSE group at eight weeks. Data are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar: 20 μm.