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. 2022 Jan 24;12:759389. doi: 10.3389/fimmu.2021.759389

Figure 5.

Figure 5

Reduced number of retinal ganglion cells in OSE mice. (A) Retinal ganglion cells were stained with RBPMS (red) on retinal cross-sections. Cell nuclei were labeled with DAPI (blue). (B) The number of retinal ganglion cells was significantly reduced in the OSE group at six weeks. This reduction was also detectable in eight-week-old animals. (C) A significant downregulation of Pou4f1 mRNA was noted in OSE mice in comparison to the control group at six and eight weeks. (D) Immunohistological staining of retinal cross-sections with GFAP (green) and DAPI (blue) was performed to label macroglia. (E) The GFAP+ area evaluation revealed no significant differences between both groups at both ages. (F) The expression of Gfap mRNA was significantly increased in OSE mice at six weeks, but no changes were detectable at eight weeks. Data are mean ± SEM for immunohistochemistry, relative values for RT-qPCR are median ± quartile ± maximum/minimum. The dotted lines in (C, F) represent the relative expression level of the control group. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer. *p < 0.05, **p < 0.01. Scale bars: 20 μm.