Table 1.
Primary and secondary antibodies used for immunohistochemistry on retina and optic nerve sections.
| Primary antibodies | Secondary antibodies | ||||
|---|---|---|---|---|---|
| Antibody | Company | Dilution | Antibody | Company | Dilution |
| Anti-C1q | Abcam | 1:200 | Donkey anti-rabbit Alexa Fluor 555 | Invitrogen | 1:500 |
| Anti-C3 | Cedarlane | 1:500 | Donkey anti-rabbit Alexa Fluor 555 | Invitrogen | 1:500 |
| Anti-GFAP | Millipore | 1:2000 | Donkey anti-chicken Alexa Fluor 488 | Jackson Immuno Research | 1:500 |
| Anti-Iba1 | Synaptic Systems | 1:500 | Donkey anti-chicken IgG Cy3 | Millipore | 1:400 |
| Anti-MAC | Thermo Scientific | 1:100 | Donkey anti-rabbit Alexa Fluor 555 | Invitrogen | 1:500 |
| Anti-RBPMS | Millipore | 1:500 | Donkey anti-rabbit Alexa Fluor 555 | Invitrogen | 1:500 |
| Anti-Tmem119 | Abcam | 1:200 | Donkey anti-rabbit Alexa Fluor 488 | Jackson Immuno Research | 1:500 |