Table 2.
Primer pairs for RT-qPCR analysis.
| Gene | Forward (F) and reverse (R) oligonucleotides | GenBank acc. no. | Amplicon size |
|---|---|---|---|
| Actb-F | ctaaggccaaccgtgaaag | NM_007393.5 | 104 bp |
| Actb-R | accagaggcatacagggaca | ||
| C1qa-F | cgggtctcaaaggagagaga | NM_007572.2 | 71 bp |
| C1qa-R | tcctttaaaacctcggatacca | ||
| C1qb-F | aggcactccagggataaagg | NM_009777.3 | 80 bp |
| C1qb-R | ggtcccctttctctccaaac | ||
| C1qc-F | atggtcgttggacccagtt | NM_007574.2 | 75 bp |
| C1qc-R | gagtggtagggccagaagaa | ||
| C3-F | accttacctcggcaagtttct | NM_009778.3 | 75 bp |
| C3-R | ttgtagagctgctggtcagg | ||
| Cd68-F | tgatcttgctaggaccgctta | NM_001291058.1 | 66 bp |
| Cd68-R | taacggcctttttgtgagga | ||
| Cfb-F | ctcgaacctgcagatccac | M57890.1 | 112 bp |
| Cfb-R | tcaaagtcctgcggtcgt | ||
| Gfap-F | acagactttctccaacctccag | NM_010277.3 | 63 bp |
| Gfap-R | ccttctgacacggatttggt | ||
| Hc-F | tgacaccaggcttcagaaagt | XM_017315669.2 | 69 bp |
| Hc-R | agttgcgcacagtcagctt | ||
| Iba1-F | ggatttgcagggaggaaaa | D86382.1 | 92 bp |
| Iba1-R | tgggatcatcgaggaattg | ||
| Masp2-F | ggcggctactattgctcct | NM_001003893.2 | 86 bp |
| Masp2-R | aacacctggcctgaacaaag | ||
| Pou4f1-F | ctccctgagcacaagtaccc | AY706205.1 | 98 bp |
| Pou4f1-R | ctggcgaagaggttgctc | ||
| Ppid-F | ttcttcataaccacaagtcaagacc | M60456.1 | 95 bp |
| Ppid-R | tccacctccgtaccacatc | ||
| Tgfb-F | aggaggtttataaaatcgacatgc | XM_006497136.3 | 65 bp |
| Tgfb-R | tgtaacaactgggcagacagttt | ||
| Tmem119-F | gtgtctaacaggccccagaa | NM_146162.3 | 110 bp |
| Tmem119-R | agccacgtggtatcaaggag | ||
| Tnfa-F | ctgtagcccacgtcgtagc | NM_013693.3 | 97 bp |
| Tnfa-R | ttgagatccatgccgttg |
The primer pairs listed in the table were used in RT-qPCR experiments. Actin (Actb) and Cyclophilin (Ppid) served as housekeeping genes. F, forward; R, reverse.