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. 2022 Jan 24;12:748303. doi: 10.3389/fimmu.2021.748303

Figure 2.

Figure 2

O. intermedium lipopolysaccharide (LPS) gives a weak inflammatory response mediated by Toll-like receptor (TLR)2 and TLR4. (A) Wild-type (WT), TLR2 knockout (KO), and TLR4 KO peritoneal macrophages were left unstimulated (NS) or were stimulated with E. coli LPS (100 ng/ml), FSL-1 (100 ng/ml), and O. intermedium LPS (1 and 10 µg/ml) for 24 h. Cytokine mRNA levels were measured by qPCR. Data ate representative of two independent experiments and expressed as the mean ± SEM. **p < 0.01, and ***p < 0.001. (B) HEK 293T, HEK 293-hTLR2/6, and HEK 293/hTLR4A-MD-2-CD14 cells were cotransfected with the pNF3ConA Luc (NF-κB) firefly reporter construct and the thymidine kinase promoter-Renilla reporter plasmid and stimulated with FSL-1 (100 ng/ml), TNF (100 ng/ml), or LPS purified from E. coli (100 ng/ml) and O. intermedium LPS (10 μg/ml). Activity of Firefly and Renilla luciferases were measured 24 h after. RLU, relative luciferase light units.