FIGURE 3.

Quantification of Aβ isoforms in 3xTg-AD mice of different age and sex. Aβ was quantified in micro-dissected hippocampi and cortices via Mesoscale Multiplex technology. (A–H) Aβ40 and Aβ42 were measured in the soluble fraction of hippocampus (A,B,E,F) and cortex (C,D,G,H), respectively, with age-related increases in Aβ40 and Aβ42 shown in hippocampus and cortex of 3xTg-AD mice between sexes. (I–P) An age-related increase in insoluble Aβ40 and Aβ42 was also observed in hippocampus (I,J,M,N) and cortex (K,L,O,P) of 3xTg-AD mice between sexes. n = 5–6 mice per genotype/age/sex. Data are represented as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.