FIG. 1.

Demonstration of the specificity of the molecular probes in PCR assay with purified bacterial DNA from different species. Amplification products of the different primer combinations were analyzed by electrophoresis on a 1.7% agarose gel. Lanes: A, 100-bp DNA ladder (GIBCO Life Technologies); B and C, primers Eco 223 and Eco 455 with E. coli (B) and P. aeruginosa (C); D and E, primers Eco 2083 and Eco 2745 with E. coli (D) and P. aeruginosa (E); F and G, primers Sau 234 and Sau 1501 with S. aureus (F) and S. epidermidis (G); H and I, primers Sau 327 and Sau 1645 with S. aureus (H) and S. epidermidis (I); J and K, primers Sag 40 and Sag 445 with S. agalactiae (J) and S. dysgalactiae (K); L and M, primers Sag 432 and Sag 1018 with S. agalactiae (L) and S. dysgalactiae (M); N, 100-bp DNA ladder (Life Technologies, Inc.); O and P, primers Sdy 105 and Sdy 386 with S. dysgalactiae (O) and S. agalactiae (P); Q and R, primers Sdy 519 and Sdy 920 with S. dysgalactiae (Q) and S. agalactiae (R); S and T, primers Spa 301 and Spa 1219 with S. parauberis (S) and S. uberis (T); U and V, primers Spa 2152 and Spa 2870 with S. parauberis (U) and S. uberis (V); W and X, primers Sub 302 and Sub 396 with S. uberis (W) and S. parauberis (X); and Y and Z, primers Sub 1546 and Sub 2170 with S. uberis (Y) and S. parauberis (Z).