Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 24;12:797390. doi: 10.3389/fimmu.2021.797390

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Nguyen, Schioppa, Tiberio, Facchinetti, Villetti, Civelli, Del Prete, Sozio, Gaudenzi, Passari, Barbazza, Sozzani, Salvi and Bosisio

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Effect of Tanimilast on primary DC activation by SCV2-RNA. cDCs (2x10⁶/ml) and pDCs (1x10⁶/ml) were pre-treated with Tanimilast (Tan, 10^-7M) and then stimulated with SCV2-RNA for 24 hours. (A, C) The surface expression of CD86, CCR7 and BDCA2 was evaluated by FACS analysis. Data are expressed as the mean ± SEM (n=3) of the percentage of positive cells (left y axis) and of the Median Fluorescence Intensity (MFI) (right y axis). (B, D) The production of TNF-α and IFN-α was evaluated by ELISA in cell-free supernatants. Cytokine expression is normalized to SCV2-RNA condition (represented as 100%). Absolute levels of SCV2-RNA induced cytokines (ng/ml) were: TNF-α= 20.92 ± 0.55; IFN-α= 169.36 ± 23.39. Data are expressed as mean ± SEM (n=3). (A–D) ^#P< 0.05 versus (-) and *P< 0.05 versus SCV2-RNA by one-way ANOVA with Dunnett’s post-hoc test.