Fig. 3.

Effect of GSK2606414 on ROS and mitochondrial function. Representative images and corresponding bar graphs of (a) DCFDA staining (b) Mitosox staining. Photographs were taken at 200× magnification (scale: 100 ​μM). Values are expressed as mean ​± ​SEM (n ​= ​3). NC: normal N2A cells, GSK 1: normal N2A cells treated with GSK260414 (1 ​μM), HG: hyperglycemic N2A cells (30 ​mM β-D glucose), HG+ ​GSK 0.5: HG cells treated with GSK260414 (0.5 ​μM), HG+ ​GSK1: HG cells treated with GSK260414 (1 ​μM), ^^P ​< ​0.01, ^^^P ​< ​0.001 v/s NC and ∗∗P ​< ​0.01, ∗∗∗P ​< ​0.001 v/s HG.