Fig. 4.

(a) Immunofluorescence staining of Mfn-2 (green) along with mitotracker (red) and (b) Representative immunoblots of proteins depicting mitochondrial function. Photographs were taken at 400× magnification (scale: 10 ​μM). Values are expressed as mean ​± ​SEM (n ​= ​3). NC: normal N2A cells, GSK 1: normal N2A cells treated with GSK260414 (1 ​μM), HG: hyperglycemic N2A cells (30 ​mM β-D glucose), HG+ ​GSK 0.5: HG cells treated with GSK260414 (0.5 ​μM), HG+ ​GSK1: HG cells treated with GSK260414 (1 ​μM), ^^P ​< ​0.01, ^^^P ​< ​0.001 v/s NC and ∗ P ​< ​0.05, ∗∗P ​< ​0.01, ∗∗∗P ​< ​0.001 v/s HG. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)