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. 2022 Jan 24;13:834296. doi: 10.3389/fphar.2022.834296

FIGURE 2.

FIGURE 2

LGL1 overexpression inhibited the proliferation and migration of VSMCs. (A) VSMCs were infected with adenovirus-expressing GFP or LGL1, then treated with PDGF-BB (20 ng/ml) for 48 h. The protein levels of Cyclin D1 and PCNA were detected by western blot (n = 3). *p < 0.05, **p < 0.01 vs. GFP + Vehicle. # p < 0.05 vs. GFP + PDGF-BB. (B, C) The mRNA levels of Cyclin D1 (B) and PCNA (C) were tested by qRT-PCR (n = 4). *p < 0.05, ***p < 0.001 vs. GFP + Vehicle. ## p < 0.01, ### p < 0.001 vs. GFP + PDGF-BB. (D) Cell proliferation measured by CCK-8 assay (n = 3). **p < 0.01, ***p < 0.001 vs. GFP + Vehicle. ## p < 0.01 vs. GFP + PDGF-BB. (E) Cell migration evaluated by scratch wound healing assay (n = 9). Wound closure (%) represented migrative ability. Scale bar: 200 μm. ***p < 0.001 vs. GFP.