FIGURE 5.

Methyltransferase‐like 14 (METTL14) negatively regulates nuclear enriched abundant transcript 1_1 (NEAT1_1) in an N6‐methyladenosine (m6A)‐dependent manner. A, m6A levels of total RNAs in tumor and matched normal tissues by RNA m6A colorimetry (n = 10). B, m6A levels of total RNAs in renal cell carcinoma (RCC) cell lines and HK‐2 cells by colorimetry. C, Correlation analysis between m6A and METTL14 in RCC samples (n = 20). D, m6A levels of METTL14 knockdown or overexpressing (OE) RCC cells by m6A dot blot assay. MB, methylene blue; NC, control. E, Methylated‐RNA immunoprecipitation (IP) assay‐quantitative PCR analysis of NEAT1_1 in METTL14 overexpressing (OE‐METTL14) RCC cells and the control (OE‐NC). F, NEAT1_1 expression in RCC cells treated with 3‐deazaadenosine (DAA) and the control. Error bars represent the mean ± SD. Student’s t test was used. *P < .05, **P < .01, ***P < .001