FIGURE 5.

Knockdown of PDRG1 inhibits the proliferation and migration of GBM cells. A, The protein level of PDRG1 in five GBM cell lines was evaluated by immunoblot. Immunoblot assay analyzed the PDRG1 protein level in U118 (B) and T98G (C) cells after PDRG1 silencing. The PDRG1 mRNA level was assessed by Q‐PCR in U118 (D) and T98G cells (E) after PDRG1 silencing. The viability of PDRG1 silenced U118 (F) and T98G (G) in 72 hours after seeding. (H) The cell cycle of PDRG1 silenced U118 and T98G was measured by flow cytometry. The migration ability of PDRG1 silenced U118 and T98G cells was assessed by Transwell assay (I, K) and wound healing assay (J, L). Data represent three independent experiments. Error bars, SD