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Coculture and neurite outgrowth measurements. (A) Coculture model of RGCs and ONHAs. Porous membrane transwell inserts (0.4 µm pore size) separated the culture system into two chambers. The RGCs were placed on the bottom of the lower chamber, and the ONHAs were placed on the membrane of the upper chamber. (B) Illustration of neurite outgrowth evaluation for total length, maximal length, root and extremity counts.
