Figure 7.

RGC apoptosis alone and with conditioned media. WT RGCs were isolated from neonatal mouse pups and cultured for 7 days with regular whole growth medium or with conditional medium from WT or S1R KO ONHAs. Scale bar, 50 µm. (A) Representative images from TUNEL assay. (B) Quantification of TUNEL-positive cells. Significantly different from control at ***P < 0.001, ****P < 0.0001. Data were analyzed using one-way ANOVA followed by the Tukey-Kramer post hoc test for multiple comparisons. Two coverslips, and eight microscopic fields per coverslip were quantified from each group of each isolation. The total number of cells analyzed was N = 400–2100. Data are from two isolations.