Immune cell population analysis of spleens, tumor-draining lymph nodes, and tumor infiltrating lymphocytes.Immune cells from the spleen, tumor-draining lymph nodes (dLNs), and tumor-infiltrating lymphocytes (TILs) were harvested on day 45 after MOC1 inoculation. Each group was treated as shown in Fig. 1.(A) The percentage of CD4+T cells, CD8+ T cells, and Gr-1+ cells in spleens, dLNs, and TILs.(B) The percentage of CD44+ CD62L+or CD44+ CD62L− in CD4+ T cells in spleens, dLNs, and TILs.(C) The percentage of CD44+ CD62L+or CD44+ CD62L− in CD8+ T cells in spleens, dLNs, and TILs.(D) The percentage of PD-1+in CD4+ or CD8+T cells in spleens, dLNs, and TILs. Bars and error bars indicate the mean and SD, respectively (*p<0.05, **p<0.01, Student's t-test).