Fig. 3.

Effects of dexamethasone on cell proliferation, survival, and surface markers of T cells and tumor cells;(A) The proliferation of EGFR_875–889-reactive HTLs (T8) and tumor cell line (HPC-92Y and HSC4) co-cultured with various concentration of dexamethasone was evaluated by MTS assay.(B) The survival of T8 and tumor cell line (HPC-92Y and HSC4) co-cultured with various concentration of dexamethasone were evaluated by flow cytometry using 7-AAD viability staining solution.(C) Expression levels of PD-1, LAG-3, and TIM3 on T8 with high-dose (3 μg/ml) or low-dose (0.3 μg/ml) dexamethasone were evaluated by flow cytometry. The panels show averages values of mean fluorescence intensity (MFI). (ns: not significant, Student's t-test). The error bars indicate standard deviations of triplicates.(D) The percentage of CD44⁺ CD62L⁺or CD44⁺ CD62L⁻ in T8 with high-dose (3 μg/ml) or low-dose (0.3 μg/ml) dexamethasone was assessed by flow cytometry.Bars and error bars indicate the mean and SD, respectively (*p<0.05, **p<0.01, Student's t-test).