Fig. 5.

IL-2 recovers the function of EGFR-reactive HTLs impaired by dexamethasone;EGFR_875–889 -specific HTLs (T8) were co-cultured with dexamethasone (3 μg/mL) for 48 h and washed with PBS. (A) Pre-treated T8 cells were rested for 5 or 10 days, and IFN-γ production in tumor cells was evaluated by ELISA. (B) Pre-treated T8 cells were co-cultured with various concentrations of IL-2 for 48 h after washing, and IFN-γ production in tumor cells was evaluated by ELISA. Bars and error bars indicate the mean and SD, respectively. The experiments were performed in triplicate (*p<0.05, Student's t-test). (C) Pre-treated T8 cells were co-cultured with IL-2 (10 U/ml or 100 U/ml) for 48 h after washing, and the percentage of CD44⁺ CD62L⁺or CD44⁺ CD62L⁻ in T8 cells was evaluated by flow cytometry.