REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
GFP Antibody Biotin Conjugated (Rabbit) (Dilute to use ∼10 nM or 500–1000× times)	Rockland Antibodies & Assays	Cat#600406215
Histidine Antibody Biotin Conjugated (Dilute to use ∼10 nM or 500–1000× times)	QIAGEN	Cat#34660
MBP Antibody Biotin Conjugated (Dilute to use ∼10 nM or 500–1000× times)	Rockland Antibodies & Assays	Cat#200406385S
Chemicals peptide, and recombinant proteins
Alconox™ Powdered Precision Cleaner	Fisher Scientific	Cat#16000104
Biotin	Sigma-Aldrich	Cat#2003993
BSA	New England BioLabs	Cat#B9000S
GdmCl	Sigma-Aldrich	Cat#200-002-3
Urea	Sigma-Aldrich	Cat#200-315-5
Proteinase-K	New England BioLabs	Cat#P8107S
SDS	Fisher Scientific	Cat#BP166-100
NaOH	Sigma-Aldrich	Cat#1310-73-2
Yeast t-RNA	Thermo Scientific	Cat#AM7119
Biotin-PEG-SVA, MW 5,000	Laysan Bio Inc.	Cat#Biotin-PEG-SVA-5000-100mg
Catalase from bovine liver	Sigma-Aldrich	Cat#C3155
Glucose Oxidase from Aspergillus niger	Sigma-Aldrich	Cat#G2133
mPEG-Succinimidyl Valerate, MW 5,000	Laysan Bio Inc.	Cat#MPEG-SVA-5000-1g
N-(2-Aminoethyl)-3-Aminopropyltrimethoxysilane	United Chemical Technologies	Cat#1760-24-3
NeutrAvidin Protein	Thermo Scientific	Cat#31000
Trolox(R), 97%, ACROS Organics™	Fisher Scientific	Cat#AC218940010
Oligonucleotides
Biotin-18merRNA: 5’-/biotin/rUrGrG rCrGrA rCrGrG rCrArG rCrGrA rGrGrC/Cy5/ -3′	Integrated DNA Technologies	N/A
U50-18mer: 5’-/Cy3/rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrG rCrCrU rCrGrC rUrGrC rCrGrU rCrGrC rCrA -3′	Integrated DNA Technologies	N/A
Biotin-18merDNA: 5’-/Cy5/GCC TCG CTG CCG TCG CCA/Biotin/-3′	Integrated DNA Technologies	N/A
18mer-G4-T9: 5′-TGG CGA CGG CAG CGA GGC TT GGG T GGG T GGG TA GGG TTT TTT TTT/Cy3/-3′	Integrated DNA Technologies	N/A
Software and algorithms
IDL	Harris Geospatial	https://www.l3harrisgeospatial.com/Software-Technology/IDL
MATLAB	MathWorks	https://www.mathworks.com/products/matlab.html
smCamera	(Roy et al., 2008)	https://cplc.illinois.edu/research/tools
Other
5 Minute® Rapid-Curing, General Purpose Adhesive Epoxy, 25 mL Tube	All-Spec	Cat#14250
Chemyx Inc fusion 200 Touch Syringe Pump	Fisher Scientific	Cat#NC0670590
ETT PTFE Tubing Size 26, 100 ft, Natural Color	Weico Wire	Cat#ETT-26
Quartz Microscope Slides	G Finkenbeiner Inc.	N/A
Scotch® 665 Permanent Double-Sided Tape, 1/2" x 250", Clear, Pack Of 3 Rolls	Office Depot	Cat#391775
TetraSpeck™ Microspheres, 0.1 μm, fluorescent blue/green/orange/dark red	Thermo Fisher Scientific	Cat#T7279
TIRF Microscope	Olympus	N/A
532 and 634-nm diode laser	Compass 315M; Coherent, Santa Clara, CA	N/A