Methylation at the sites of genes known to be expressed at the different developmental stages
The PGC-associated Tet and Dazl, spermatogonia-associated Id4, prospermatogonia-associated Stra8, and spermatid-associated protamines Prm1 and Prm2 are presented. The relative level of DNA methylation of the chromosomal sites of each developmental stage-specific gene. The color insert identifies the specific genes. The relative DNA methylation was determined as mean RPKM read depth.