FIG. 2.

Specific amplification of M. tuberculosis rpoB DNA by first-round PCR (TB1-TB2 primer set). The Southern blot shows that the PCR product (205 bp) was amplified from only M. tuberculosis by the first-round PCR. (A and B) Lanes: 1, M. tuberculosis H37Rv; 2, clinical isolate of M. tuberculosis; M, φX174/RF DNA/HaeIII digest; 3, M. avium; 4, M. fortuitum; 5, M. gastri; 6, M. gordonae; 7, M. intracellulare; 8, M. kansasii; 9; M. malmoense; 10, M. nonchromogenicum; 11, M. phlei; 12, M. scrofulaceum; 13, M. simiae; 14, M. smegmatis; 15, M. terrae. (C and D) Lanes 16, M. triviale; 17, M. vaccae; 18, M. chelonae; 19, M. szulgai; 20, M. ulcerans; 21, Rhodococcus equi; 22, Rhodococcus erythropolis; 23, Rhodococcus rhodochrous; 24, Nocardia otitidiscaviarum; 25, Nocardia nova; 26, Corynebacterium glutamicum; 27, Corynebacterium diphtheriae; 28, Neisseria meningitidis; 29, Haemophilus influenzae.