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Amplification of rpoB DNA by nested PCR using serially diluted M. tuberculosis DNA as templates. Amplified products are observed only in the lanes that used more than 10 fg of template DNA. Lanes: M, φX174/RF DNA/HaeIII digest; 1, 10 ng; 2, 1 ng; 3, 100 pg; 4, 10 pg; 5, 1 pg; 6, 100 fg; 7, 10 fg; 8, 1 fg; 9, negative control without DNA.
