FIG. 4.

Comparison of PCR-SSCP and nested PCR-SSCP performed with three sputum specimens, N3104 (His₅₂₆ [CAC]→Arg [CGC]), N3928 (wild type), and N3983 (Ser₅₃₁ [TTG]→Leu [TCG]). Patterns of conventional PCR-SSCP using the TR9-TR8 primer set showed more than two bands (lanes P). They were unusual in PCR-SSCP analysis performed with culture. However, nested PCR-SSCP showed only two bands by which the rifampin resistance of M. tuberculosis could be determined as other reports (lanes N). H, M. tuberculosis H37Rv.