Nested PCR-SSCP patterns of rifampin-resistant strains (lanes 1 to 9) identified in this study. Lanes: H, M. tuberculosis H37Rv; 1, N3523 (Gln513→Pro); 2, N2893 (Asp516→Val); 3, N2910 (His526→Tyr); 4, N3501 (His526→Asp); 5, N2902 (His526→Arg); 6, N2895 (Ser531→Leu); 7, N3522 (Ser531→Met); 8, N2877 (Leu533→Pro); 9, N3471 (Met515, Asp516, Gla517, Asn518→Ile, Ala, Asn, Tyr).