TABLE 2.
Rifampin susceptibility of M. tuberculosis in sputa determined by conventional PCR-SSCP and nested PCR-SSCP analyses
| Method | Result | No. of sputum samples found by rifampin susceptibility testinga to be:
|
|
|---|---|---|---|
| Susceptible (n = 33) | Resistant (n = 23) | ||
| PCR-SSCP | Susceptible | 25 | 0 |
| Resistant | 0 | 20 | |
| Not determinedb | 8 | 3 | |
| Nested PCR-SSCP and DNA sequencing | Susceptible | 33 | 0 |
| Resistant | 0 | 23 | |
| Not determined | 0 | 0 | |
a
Agar dilution method performed with cultures from the same sputa used for SSCP analysis.
b
SSCP patterns could not be interpreted due to no amplification or more than three bands.