TABLE 3.

Comparison of results determined by three different methods to determine rifampin susceptibility of M. tuberculosis in sputa and their culture isolates

Specimen no.	Clinical specimen designation	Resistance toa:				Result from:
		RIF	INH	STR	EMB	Conventional PCR-SSCP or direct sequencingb	Nested PCR-SSCP or direct sequencingb	DNA sequencing
1	N2838	S	S	S	S	Wild type	Wild type	No mutation
2	N2872	S	S	S	S	Wild type	Wild type	No mutation
3	N2877	R	R	S	R	Abnormal	Abnormal	Mutation
4	N2893	R	R	S	R	Abnormal	Abnormal	Mutation
5	N2895	R	R	S	S	Not determinedc	Abnormal	Mutation
6	N2902	R	R	S	R	Abnormal	Abnormal	Mutation
7	N2903	R	R	S	R	Abnormal	Abnormal	Mutation
8	N2904	S	S	S	S	Wild type	Wild type	No mutation
9	N2910	R	R	R	R	Abnormal	Abnormal	Mutation
10	N2984	R	R	S	S	Abnormal	Abnormal	Mutation
11	N2994	S	S	S	S	Not determined	Wild type	No mutation
12	N2999	S	S	S	S	Wild type	Wild type	No mutation
13	N3000	S	S	S	S	Wild type	Wild type	No mutation
14	N3012	S	S	S	S	Wild type	Wild type	No mutation
15	N3085	S	S	S	S	Wild type	Wild type	No mutation
16	N3096	S	S	S	S	Wild type	Wild type	No mutation
17	N3104	R	R	R	S	Ambiguousd	Abnormal	Mutation
18	N3105	S	S	S	S	Wild type	Wild type	No mutation
19	N3106	S	S	S	S	Wild type	Wild type	No mutation
20	N3426	S	S	S	S	Wild type	Wild type	No mutation
21	N3428	S	S	S	S	Wild type	Wild type	No mutation
22	N3440	R	R	S	R	Not determined	Abnormal	Mutation
23	N3448	S	S	S	S	Not determined	Wild type	No mutation
24	N3471	R	R	S	S	Abnormal	Abnormal	Mutation
25	N3474	R	R	S	S	Abnormal	Abnormal	Mutation
26	N3496	R	R	R	R	Abnormal	Abnormal	Mutation
27	N3500	S	S	S	S	Wild type	Wild type	No mutation
28	N3501	R	R	S	S	Abnormal	Abnormal	Mutation
29	N3507	S	S	S	S	Wild type	Wild type	No mutation
30	N3522	R	R	S	R	Abnormal	Abnormal	Mutation
31	N3523	R	R	S	S	Abnormal	Abnormal	Mutation
32	N3524	R	R	S	R	Abnormal	Abnormal	Mutation
33	N3536	R	R	S	S	Abnormal	Abnormal	Mutation
34	N3606	S	S	S	S	Wild type	Wild type	No mutation
35	N3874	R	R	S	S	Abnormal	Abnormal	Mutation
36	N3878	S	S	S	S	Wild type	Wild type	No mutation
37	N3903	S	S	S	S	Not determined	Wild type	No mutation
38	N3912	S	S	S	S	Not determined	Wild type	No mutation
39	N3914	S	S	S	S	Wild type	Wild type	No mutation
40	N3924	S	S	S	S	Wild type	Wild type	No mutation
41	N3928	S	S	S	S	Ambiguous	Wild type	No mutation
42	N3983	R	R	S	S	Ambiguous	Abnormal	Mutation
43	N3986	R	R	S	R	Abnormal	Abnormal	Mutation
44	N3990	S	S	S	S	Not determined	Wild type	No mutation
45	N3992	S	S	S	S	Wild type	Wild type	No mutation
46	N3993	S	S	S	S	Wild type	Wild type	No mutation
47	N4025	R	R	S	R	Abnormal	Abnormal	Mutation
48	N4030	R	R	R	R	Abnormal	Abnormal	Mutation
49	N4062	S	S	S	S	Wild type	Wild type	No mutation
50	N4064	S	S	S	S	Not determined	Wild type	No mutation
51	N4069	S	R	S	R	Wild type	Wild type	No mutation
52	N4115	R	R	S	S	Abnormal	Abnormal	Mutation
53	N4116	S	S	S	S	Wild type	Wild type	No mutation
54	N4123	S	S	S	S	Wild type	Wild type	No mutation
55	N4159	S	S	S	S	Wild type	Wild type	No mutation
56	N4165	S	S	S	S	Wild type	Wild type	No mutation

^aRIF, rifampin; INH, isoniazid; STR, streptomycin; EMB, ethambutol; S, susceptible; R, resistant. 

^bWild type or Abnormal, identical SSCP patterns and sequences or different from that of reference strain, respectively. 

^cProducts were poorly amplified for the SSCP analysis. 

^dThe extra bands originating from other bacteria which may coexist with M. tuberculosis interfered with SSCP analysis.