TABLE 3.

Nucleic acid targets for primers and probes

Assay	Purpose	Targetb	Product size	Primer name	Sequence and label(s) (5′→3′)d
ISH	Probe	L. pneumophila 16S M36026a		P1a	ATC TGA CCG TCC CAG GTT
ISH	Probe	L. pneumophila 16S M36026		P2	AGC TTT CAT CCA AAG ATA
PCR	Primer	L. pneumophila mipS72442	24	LPmipAf	ACC GAA CAG CAA ATG AAA GA
PCR	Primer	L. pneumophila mipS72442	124	LPmipAr	AAC GCC TGG CTT GTT TTT GT
PCR	Primer	Legionella species 5S X05081	105	L5Fc	ACT ATA GCG ATT TGG AAC C
PCR	Probe	Legionella species 5S X05081		L5Pc	RCAT GAG GAA GCC TCA CAC TAT CAP
PCR	Primer	Legionella species 5S X05081	105	L5RBc	GGC GAT GAC CTA CTFT TC
PCR	Probe	L. pneumophila mipS72442		LPP1	AAC AAG TTT CAG AAA GAT TTG ATG GCA AAGF
PCR	Probe	L. pneumophila mipS72442		LPP2	RGTA CTG CTG AAT TCA ATA AGT AAG CGG ATGP

^aPreviously described, as LEGPNE1, by Grimm et al. (19). 

^bGenBank accession numbers are given. 

^cModified from sequences described by Mahbubani et al. (35). 

^dR, LC-Red 640 fluorescent dye; F, LC fluorescein dye; P, phosphate.