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. 2022 Jan 24;10:722722. doi: 10.3389/fbioe.2022.722722

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Copyright © 2022 Wang, Du, Zhang, Xu, Zhang, Jia and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A,B) Relative eGFP copy number and mRNA expression level in long-term stable transfected cells. Fluorescent quantitative PCR was used to analyze eGFP gene copy numbers and mRNA expression. Relative copy numbers and mRNA expression levels were calculated using the 2^−ΔΔCt method and were normalized to the BGH poly A vector whose value was set to 1. (C) Correlation analysis between the relative eGFP copy (blue diamond), mRNA expression (red square) and relative eGFP expression in long-term stable transfected CHO cells. Results were obtained from three independent experiments. The standard error of the mean (S.E.M.) was indicated.