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. Author manuscript; available in PMC: 2022 Dec 23.
Published in final edited form as: J Med Chem. 2021 Dec 2;64(24):18054–18081. doi: 10.1021/acs.jmedchem.1c01476

Figure 4.

Figure 4.

Effects of GDC-0068-based VHL-recruiting compounds on degrading AKT and inhibiting downstream signaling. BT474 cells were treated with GDC-0068 (1 μM); VHL-1 (1 μM); pomalidomide (POM, 1 μM); compounds 3–11 at 1, 5, and 10 μM; or compounds 12–13 at 1, 3, and 10 μM for 24 h. The cell lysates were analyzed by western blotting to examine the protein levels of total AKT (T-AKT) and phosphorylated AKT at serine 473 (P-AKT (S473)), and downstream signaling inhibition of P-PRAS40 (T246) and P-S6 (S240/244). β-Actin was used as the loading control.