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. Author manuscript; available in PMC: 2022 Dec 23.
Published in final edited form as: J Med Chem. 2021 Dec 2;64(24):18054–18081. doi: 10.1021/acs.jmedchem.1c01476

Figure 5.

Figure 5.

Effects of GDC-0068-based CRBN-recruiting compounds on degrading AKT and inhibiting downstream signaling. BT474 cells were treated with GDC-0068 (1 μM), VHL-1 (1 μM), POM (1 μM), or indicated compounds at 1, 5, and 10 μM for 24 h. The cell lysates were analyzed by western blotting to examine the protein levels of T-AKT, P-AKT (S473), P-PRAS40, (T246) and P-S6 (S240/244). β-Actin was used as the loading control.