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. 2022 Jan 24;12:807628. doi: 10.3389/fgene.2021.807628

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Copyright © 2022 Liu, Feng, Zeng, He, Gong and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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VPS9D1-AS1 can be a molecular sponge for miRNA-30a-5p. (A) LncATLAS database was used for subcellular localization analysis of VPS9D1-AS1. (B) Subcellular fractionation and qRT-PCR were introduced to analyze relative expression of VPS9D1-AS1 in the nuclear and cytoplasmic of A549 cells. (C) Volcano plot of differentially expressed miRNAs in TCGA-LUAD. Red indicates increased miRNAs, and green represents decreased miRNAs. (D) Intersection of miRNAs that have binding sites with VPS9D1-AS1 and differentially downregulated miRNAs in LUAD in LncBase database. (E) The correlation of VPS9D1-AS1, miRNA-30a-5p, miRNA-308c, and miRNA-378a-3p levels in LUAD tissue. (F) Box plot of miRNA-30a-5p level. Blue plot indicates normal tissue, and red plot indicates LUAD tissue. (G) Survival analysis of high- and low-expression groups with the median value of miRNA-30a-5p as the cutoff value. (H) Expression of miRNA-30a-5p in BEAS-2B and A549, Calu-3, H1975, and SPC-A-1 cells (* represents p < 0.05 in comparison with BEAS-2B group). (I) Effect of overexpressing or silencing VPS9D1-AS1 on miRNA-30a-5p level in A549 and H1975 cells (* represents p < 0.05 in comparison with the negative control group). (J) Interaction between VPS9D1-AS1 and miRNA-30a-5p. IgG was a negative control (* represents p < 0.05 in comparison with IgG group). (K) Binding of VPS9D1-AS1 and miRNA-30a-5p (* represents p < 0.05 in comparison with the negative control group). All assays were repeated three times in triplicate.