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. 2022 Jan 24;12:807628. doi: 10.3389/fgene.2021.807628

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Copyright © 2022 Liu, Feng, Zeng, He, Gong and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MiRNA-30a-5p restores the effect of VPS9D1 on promoting cell proliferation, migration, and invasion of LUAD. (A) Overexpression efficiency of transfecting miRNA-30a-5p-mimic on miRNA-30a-5p level in A549 and H1975 cells. (B) The impact of simultaneous overexpression of VPS9D1-AS1 and miRNA-30a-5p on the viability of the LUAD cells. (C) Effect of simultaneous overexpression of VPS9D1-AS1 and miRNA-30a-5p on the colony formation ability of the LUAD cells. (D) The impact of simultaneous overexpression of VPS9D1-AS1 and miRNA-30a-5p on migratory ability of the LUAD cells (40 ×). (E) The impact of simultaneous overexpression of VPS9D1-AS1 and miRNA-30a-5p on invasive ability of the LUAD cells (100 ×). * represents p < 0.05 when oe-NC + NC-mimic group was compared to oe-VPS9D1-AS1 + NC-mimic group and when oe-VPS9D1-AS1 + NC-mimic group was compared to oe-VPS9D1-AS1+miR-30a-5p-mimic group. All assays were repeated three times in triplicate.