Table 3.

The qRT-PCR primers for each species examining CamCYC2A and CamCYC2B gene expression patterns

		Efficiency (%)
	Lobelia erinus
CamCYC2A	CYC2 37F 5′-GCTAGTAAAACCCTTGATTGGCT-3′	82.7
	CYC2A 314R 5′-GCCCTGGACTCTTTTGCAAAGT-3′
CamCYC2B	CYC2 37F 5′-GCTAGTAAAACCCTTGATTGGCT-3′	81.8
	CYC2B 291R 5′-GCGATGAGATGCAGGTTTATAACTG-3′
CamActin	Le-act1046F 5′-ATCCACGARACSACCTACAACT-3′	86.0
	Le-act1216R 5′- MACCACCTTAATCTTCATGCTGCT-3′
	Lobelia siphilitica
CamCYC2A	ls CYC2A-37F 5′-TTCGACAAAGCTAGTAAAACTCTTGATTGG-3′	81.6
	Ls CYC2A 264R 5′-TTTCTCTTTGGCTCTCGTTGTAGC-3′
CamCYC2B	ls CYC2B-47F 5′-CTAGTAAAACCCTTGATTGGCTTTTCAC-3′	87.0
	Ls CYC2B 298R 5′-CTAGGCGATGAGATGCAGGTTTATAAC-3′
CamActin	Le-act477F 5′-AGATYTGGCATCAYACTTTCTACA-3′	89.0
	Le-act729R 5′- CCTTCGTARATTGGAACCGTGTG-3′
	Lithotoma axillaris
CamCYC2A	Ls CYC2A F41 5′-ACAAAGCTAGTAAAACTCTTGATTGGCT-3′	89.3
	ISCYC2A 260R 5′-TTCTCTTTGGCGCTCGATGTAGCTG-3′
CamCYC2B	ISCYC2B 38F 5′-TTGACAAAGCTAGTAAAACCCTTGATTGG-3′	92.8
	ISCYC2B 203R 5′-GCTCCTTCATTTTGTTCAGCTGC-3′
CamActin	Le-act1046F 5′-ATCCACGARACSACCTACAACT-3′	86.0
	Le-act1216R 5′-MACCACCTTAATCTTCATGCTGCT-3′
	Lobelia polyphylla
CamCYC2A	LP CYC2AF1a 5′-TCGACAAAGCTAGTAAAACTCTTGATTGG-3′	89.6
	LP CYC2A R4 5′-TTTGCAAGATAAAGTGCAGGTTTATACG-3′
CamCYC2B	Ls CYC2B F43 5′-AAAGCTAGTAAAACCCTTGATTGGCT-3′	85.7
	LP CYC2B R1 5′-TTTGTGCTCTCATCGTTTTCGCTTCAC-3′
CamActin	Le-act477F 5′-AGATYTGGCATCAYACTTTCTACA-3′	89.0
	Le-act729R 5′-CCTTCGTARATTGGAACCGTGTG-3′

The annealing temperature for all primers is 60 ℃