Figure 5.

A) A plot of the maximum intensity of mOX063-d24-GB1 vs. time in 10 times excess of ascorbate or cytosol extracted from Xenopus laevis oocytes. The height of the vertical bars represents the RMSD in the CW spectrum. B) DQC time trace of mOX063-d24-GB1 at 80 K Q-band before and after artifact subtraction. C) Distance distribution (most probable distance of 3.6 nm) extracted from the artifact-subtracted time-domain signal using DeerAnalysis. The gray region represents the error obtained from the validation function.