Fig. 1.

Schematic of the RT-QuIC buffer discovery phase to evaluate α-synuclein seeding in MSA. Step 1: Samples containing α-synuclein seeds were prepared from substantia nigra of PD or cerebellum of MSA patients. Step 2: The samples were incubated with 168 reaction buffers and subjected to cycles of shaking and resting at 37 °C. Step 3: ThT output was measured at multiple timepoints over 48 h. Step 4: Results were analyzed and heatmaps were generated to determine the optimal conditions to discriminate MSA-derived samples from PD-derived samples. Designed with Biorender.com