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. 2022 Feb 7;11:7. doi: 10.1186/s40035-022-00283-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Conformational discrimination of α-synuclein strains in PD and MSA brains. a Representative immunohistochemistry image for aggregated α-synuclein in the cerebellum white matter from a MSA patient showing glial cytoplasmic inclusions. b Representative immunohistochemistry image for aggregated α-synuclein in the substantia nigra (SN) from a PD patient showing Lewy bodies and Lewy neurites. c Representative total α-synuclein (Syn-1 clone) immunoblots showing the thermolysin (TL) digestion of brain extracts from the cerebellum and the SN of a MSA and a PD patient, respectively. d, e Conformational stability assays for α-synuclein aggregates in brain extracts from patients with MSA or PD. Representative α-synuclein immunoblots (d) and the resultant denaturation curves (e) are shown. The curves depict mean residual α-synuclein values following treatment with the indicated concentrations of GdnCl. Higher GdnCl₅₀ values were obtained for α-synuclein aggregates in the patient with PD than that in the patient with MSA