Fig. 4.

Bias estimates for raw and corrected data sets. A Bray-Curtis dissimilarity of relative abundance data of 16S rRNA gene amplicon sequencing (NGS) to HT-qPCR relative abundance data (reference). Depicted are the dissimilarities for the raw and the corrected Lactiplantibacillus plantarum group data sets without and with gene copy number normalization (GCN) for the 16S rRNA gene. B Bias point estimates ± two geometric standard errors before and after the correction of the assignments for the L. plantarum group species. C Bias point estimates ± two geometric standard errors after the correction of the assignments for the L. plantarum group species, with (corr. GCN) and without (corr.) 16S rRNA gene copy number correction