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. 2022 Feb 7;21:37. doi: 10.1186/s12943-022-01517-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Knockout of HNRNPU regulated the BUC cell proliferation, apoptosis, and migratory through regulating NF1 expression. T24 cells were transfected with sgHNRNPU, with or without the NF1 knockdown, and then treated with or without cisplatin. (A) T24 cell viability was measured using CCK-8 assay. (B) Cell invasion of T24 cancer cells was determined by transwell assay. (C) Cell migration of T24 cancer cells was determined by wound healing assay. (D) The cell cycle of T24 cells with different treatments were detected by flow cytometry. (E) The apoptotic rate was examined by flow cytometry. The data indicate mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001