Figure 6.

RP81-MNP decreases inflammatory cells and preserves proximal tubular cells. (A) UMAP plots show the renal cell populations in the kidneys of RNLS KO CKD mouse treated with empty MNP (control, on the left) or MNP loaded with RP81 (RP81-MNP, on the right). The plots are colored by orthogonally generated clusters labeled by manual cell type annotation. (B and C) Proportions of each cell type in control and RP81-MNP samples. (D–H) Volcano plots showing genes whose expression was significantly (Log2 fold change, P<0.05) deregulated by RP81-MNP treatment in kidney PT cells in S1 segment (D), S2/S3 segment (E), and stressed and injured PT (F and G, respectively), and in inflamed macrophages (H). Genes shown in blue on the left of volcano plots are downregulated by RP81-MNP and genes in red on the right of volcano plots are upregulated by RP81-MNP treatment. PT, proximal tubule; TAL, thick ascending limb; DCT, distal convoluted tubule; CNT, connecting tubule; CD, collecting duct; PC, principal cell; IC, intercalated cell; JGA: juxtaglomerular apparatus; VSMC, vascular smooth muscle cell; DC, dendritic cell.